LOCUS       40924_31740       12881 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB326 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12881)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12881)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 12881)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12881)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        484..514
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             568..1224
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1569..1871
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1915..2039)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    2053..2070
                     /label=GGSGGS linker
                     /note="GGSGGS linker"
     CDS             2077..2763
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="ELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALKLV
                     GGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYCDL
                     PSKLGHK"
     terminator      2788..3040
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3074..3090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3098..3114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3122..3152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3167..3188)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(3273..3448)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3456..5492)
                     /codon_start=1
                     /gene="mALS"
                     /product="acetolactate synthase"
                     /label=mALS
                     /note="from Marchantia polymorpha; contains triple
                     amino-acid subustitutions (P207S/R208S/W582L)"
                     /protein_id="BAS49969.1"
                     /translation="MASISGCAAVPLSIASLEKVQPSARSCCKGAKELKVRSGFFSGAA
                     LRGHAYNTSRKTERSLEVKAQAVSEKIQSAVDVSGPRLSSGKSLVAPTPSDFQSRYKDD
                     EPRKGADILVEALEREGIRTVFAYPGGASMEIHQALTRSSSIRNILCRHEQGEIFAAEG
                     YAKSSGRVGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVSSRMIGTDAFQETPIV
                     EVTRSITKHNYLVMNIDDIPRVIREAFFLAASGRPGPVLVDIPKDVQQQMAIPDWSQPM
                     QLKGYLQRLPRPPEPTLIEQIIRLISSSKKPVVYSGGGCNHASEELREFVELTGIPITS
                     TLMGLGAYPESDPLSLKMLGMHGTVYANYAVDQADLLLAFGVRFDDRVTGKLETFASRS
                     SIVHIDIDPAEIGKNKQPHVSMCADVKPALSEINALLKSQKRTFDFSNWVAELEEQKQK
                     WPMKFPVFEDAIAPQSAIQTLYELTGGNAIISTGVGQHQMWAAQWFKYNEPRRWLTSGG
                     LGAMGFGLPSALGAAATQPDIPVIDIDGDGSFVMNVQELATIFVEKLPVKIMILNNQHL
                     GMVVQLEDRFYKANRAHTYLGDPDHEEQIYPDFCTVADGFKVPNARVVKKDELRAAIQT
                     MLDSPGPYLLDVIVPHQEHVLPMIPGGASFKEIITEGDGRTIY"
     gene            complement(3456..5492)
                     /gene="mALS"
                     /label=mALS
     promoter        complement(5532..6208)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(6403..6427)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6948..7736
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7985..8573
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8759..8899)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9243..9437)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9506..10576)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11008..11634)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagcg ctggaggctc cggtgggagc atgagcgagc tgattaagga gaacatgcac
     2101 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
     2161 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
     2221 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc
     2281 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg
     2341 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     2401 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
     2461 cctgtgatgc agaagaaaac actcggctgg gaggccaaca ccgagatgct gtaccccgct
     2521 gacggcggcc tggaaggcag aagcgacatg gccctgaagc tcgtgggcgg gggccacctg
     2581 atctgcaact tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     2641 ggcgtctact atgtggacca cagactggaa agaatcaagg aggccgacaa agagacctac
     2701 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     2761 aagtgagctt agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     2821 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     2881 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     2941 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     3001 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt tccggaacca
     3061 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3121 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3181 tcacattagg ctgaattagg cgcgcctatt tctgaattca acacattgcg gacgttttta
     3241 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     3301 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     3361 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaaattc ccttatctgg
     3421 gaactactca cacattatta tggagaaact cgaagttagt atatagtcct tccatcaccc
     3481 tctgtgataa tctccttaaa acttgctccc ccaggaatca taggcagaac gtgctcctgg
     3541 tggggaacta tcacatccag taaataagga ccgggagaat ctagcatagt ttgaattgct
     3601 gctcttagtt catccttctt caccacacgc gcatttggca ccttgaaacc gtctgcaact
     3661 gtacagaagt cggggtagat ctgctcctcg tgatctggat cacctaagta agtatgtgct
     3721 ctgttggcct tgtagaatct atcctccaat tgtacgacca ttcctagatg ttggttgttt
     3781 aagatcatga tcttcacggg taacttctcc acaaaaattg tagccaactc ctgtacgttc
     3841 atcacaaagc taccatcgcc atcgatgtcg atcacaggaa tatctggttg agttgcggca
     3901 gcccccaaag cagaaggaag tccgaatccc atggctccaa gacctccaga tgtcaaccaa
     3961 cgccgcggtt cgttgtactt gaaccactgg gctgcccaca tttgatgttg gccaacacca
     4021 gtagatatga tggcatttcc accagtgagt tcgtacagcg tctgtatggc agattgagga
     4081 gcaatggcat cctcgaatac gggaaacttc attggccatt tctgcttttg ttcttctagt
     4141 tctgccaccc agttggagaa gtcgaaagtc ctcttttgac tcttcaagag ggcattgatt
     4201 tcacttagcg caggtttcac atcagcacac atggacacat ggggctgttt gttctttcca
     4261 atttcagcag ggtcaatatc gatatgaacg atgctggacc tgctagcaaa agtctccagt
     4321 ttaccagtca ctcgatcgtc gaaacgcact ccaaatgcca acaacagatc tgcttggtcc
     4381 acggcgtagt tggcgtaaac agttccatgc attccaagca ttttcaaact taatggatca
     4441 gactcgggat acgctcccaa acccattaaa gtcgatgtga taggaattcc ggtaagctcg
     4501 acaaattccc tcaactcttc gctggcgtga ttgcaacctc ctccagaata cactacaggc
     4561 ttcttagaac tagaaatgag tcttataatc tgctcgatta acgttggttc cggtggccga
     4621 ggcagtcgtt gtaagtagcc cttcagttgc attggctgag accaatctgg aatagccatt
     4681 tgctgttgca cgtccttggg aatatctacc aagactgggc ccggcctacc tgaagctgcc
     4741 aaaaagaaag cctccctgat tacgcgaggg atgtcatcaa tgttcataac aaggtaattg
     4801 tgcttggtga tcgatctcgt aacctccaca attggagttt cttgaaaagc atcggtacca
     4861 atcatcctac tggatacttg acctgtaatg gccactagtg gcacactgtc caagagggca
     4921 tcagcgagcc cggtaacaag attggtcgct ccaggaccgg atgtggctat acaaacccct
     4981 actcgtccag acgatttagc ataaccttca gcagcgaaaa tctccccctg ctcgtggcga
     5041 cagagaatgt tgcggatgct gctggaacgt gtaagagcct ggtgaatctc catagacgct
     5101 ccgccgggat aagcgaacac cgttctaatc ccttctcgct caagagcctc caccaaaatg
     5161 tcggctcctt tgcggggctc gtcatccttg tagcgagact gaaagtcgga cggggttggt
     5221 gcaacgagtg acttcccgct gctaagccgt ggtccactca catcgactgc agactggatc
     5281 ttctcgctaa cagcttgggc tttcacttcc agtgaacgct cagtcttccg agaggtgttg
     5341 tatgcatgtc ccctcagagc ggctcccgag aagaaaccac tcctcacctt tagctcttta
     5401 gctcccttgc aacaagacct tgccgatggc tgcaccttct ccaggctcgc tatgctcaaa
     5461 ggcacagctg cgcatccact aattgacgcc atgctcgaga gagatagatt tgtagagaga
     5521 gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg
     5581 tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa
     5641 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     5701 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     5761 tcgcaattat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     5821 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     5881 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     5941 tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     6001 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     6061 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
     6121 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
     6181 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
     6241 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca
     6301 atacgcaaac cgaattcaga aataaattca gcctaattcg gcgttaattc agtacattaa
     6361 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
     6421 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
     6481 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
     6541 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
     6601 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
     6661 gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     6721 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     6781 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     6841 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     6901 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg
     6961 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     7021 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     7081 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     7141 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     7201 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     7261 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     7321 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     7381 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     7441 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     7501 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     7561 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     7621 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     7681 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat
     7741 gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
     7801 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     7861 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     7921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8101 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8641 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8701 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8761 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     8821 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     8881 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8941 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     9001 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     9061 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     9121 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     9181 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     9241 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     9301 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     9361 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     9421 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     9481 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     9541 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     9601 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     9661 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     9721 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     9781 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     9841 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     9901 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     9961 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
    10021 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
    10081 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
    10141 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
    10201 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
    10261 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
    10321 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
    10381 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
    10441 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
    10501 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
    10561 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
    10621 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
    10681 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
    10741 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
    10801 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
    10861 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
    10921 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
    10981 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
    11041 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
    11101 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
    11161 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
    11221 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
    11281 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
    11341 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
    11401 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    11461 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    11521 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    11581 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    11641 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    11701 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    11761 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    11821 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
    11881 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
    11941 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
    12001 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
    12061 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
    12121 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
    12181 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
    12241 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
    12301 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
    12361 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
    12421 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
    12481 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
    12541 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
    12601 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
    12661 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
    12721 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
    12781 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
    12841 gtggttggca tgcacataca aatggacgaa cggataaacc t
//
